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Immunology and Infection
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Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

DOI:
10.3791/51301-v

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11:40 min

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March 27, 2014

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, , , , ,
1UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology,University of Alabama at Birmingham

Chapters

  • 00:05Title
  • 02:33Cell Isolation and Plating
  • 08:02Results: Representative Bioenergetic Index Analyses
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.

Tags

Keywords: BioenergeticsOxidative BurstLeukocytesPlateletsMitochondrial DysfunctionAtherosclerosisDiabetesSeptic ShockNeurodegenerative DiseasesHyperglycemiaInflammationMagnetic Bead IsolationCell AdhesionMicroplate RespirometryExtracellular Flux AnalyzerMonocytesLymphocytesNeutrophilsReactive Oxygen Species

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