Proliferation Apoptosis Studies
Proliferation Apoptosis Studies - Related Videos
03:48
Choroid Sprouting Assay to Study Ocular Microvascular Proliferation Ex Vivo
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2025
This video demonstrates the development of an ex vivo model of choroidal sprouting to study ocular microvascular angiogenesis. The assay involves culturing mouse eye explants containing the choroid tissue attached to the sclera and retinal pigment epithelium under specific conditions that promote choroidal microvascular sprouting.
01:30
Apoptosis
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2023
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.
07:42
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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Cited by 15 •
2011
In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
06:58
Synthesis of an In vivo MRI-detectable Apoptosis Probe
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Cited by 3 •
2012
Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.
08:27
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
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Cited by 20 •
2014
Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.
FAQs
The two measurements provide complementary views of cell-number regulation. Cell-cycle progression indicates how actively cells are being produced, whereas apoptosis-related molecular changes indicate how cells are being eliminated. Examining both processes helps researchers determine whether altered tissue structure reflects excessive division, insufficient cell removal, or disruption of both sides of the balance.
Caspases function as intracellular signaling components that activate the controlled molecular changes associated with apoptosis. Their activation helps distinguish regulated cell dismantling from a general loss of cellular viability. Measuring apoptosis through this signaling process allows studies to connect observed cell elimination with an organized biological mechanism relevant to tissue health and disease progression.
Cell-cycle progression provides evidence about how cells move through the stages associated with division. This makes it possible to evaluate changes in cell production rather than relying only on the final number of cells present. In combination with apoptosis measurements, cell-cycle information helps explain how interventions or disease states alter tissue maintenance and structure.
A study first selects a biological comparison, such as normal and diseased tissue or treated and untreated material. Researchers then assess proliferation through cell-cycle progression and analyze apoptosis through caspase-linked, controlled molecular changes. Comparing these complementary results shows how the tested condition affects cell production, cell elimination, or the balance between them.
These studies help determine whether an anticancer treatment changes the processes that control cell number. Researchers can compare treated and untreated systems to assess effects on cell-cycle progression and regulated cell elimination. The combined outcome indicates whether an intervention may alter disease-associated tissue behavior by influencing proliferation, apoptosis, or both processes together.
Developmental and regenerative systems require coordinated control of cell production and removal to establish or restore tissue structure. By measuring proliferation alongside apoptosis, researchers can examine how this coordination changes during development, repair, or regeneration. The results help relate cellular behavior to tissue organization and identify disruptions that may affect biological health.