Protozoa

Protozoa are diverse, mostly microscopic eukaryotic organisms that often live as single cells in aquatic environments, soils, or other organisms, making them important subjects in biology. Their cells contain a nucleus and specialized structures that support movement, feeding, waste removal, and reproduction; many move using cilia, flagella, or temporary extensions called pseudopodia. Protozoa obtain nutrients by ingesting particles, absorbing dissolved substances, or living in symbiosis, while some form resistant cysts when conditions become unfavorable. Studying protozoa helps explain cellular organization, microbial ecology, nutrient cycling, disease transmission, and interactions between organisms, including parasitic infections and beneficial relationships in ecosystems.

Protozoa - Related Videos

Research

JoVE Journal - Biology

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

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Cited by 11 •

2010

We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

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Cited by 5 •

2020

The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

Research

JoVE Journal - Biology
Free Sample

Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community

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Cited by 1 •

2007

Jared Leadbetter takes us for a nature walk through the diversity of life resident in the termite hindgut - a microenvironment containing 250 different species found nowhere else on Earth. Jared reveals that the symbiosis exhibited by this system is multi-layered and involves not only a relationship between the termite and its gut inhabitants, but also involves a complex web of symbiosis among the gut microbes themselves.

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

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Cited by 18 •

2007

This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

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Cited by 9 •

2020

Here, we provided a method to achieve stable transfection of chicken Eimeria parasites by nucleofecting sporozoites or second-generation merozoites. Genetically modified eimerian parasites expressing heterologous antigenic genes could be used as vaccine delivery vehicles.

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