Scanning Probe Microscopy
Scanning Probe Microscopy - Related Videos
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
10:53
Scanning-probe Single-electron Capacitance Spectroscopy
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2013
Scanning-probe single-electron capacitance spectroscopy facilitates the study of single-electron motion in localized subsurface regions. A sensitive charge-detection circuit is incorporated into a cryogenic scanning probe microscope to investigate small systems of dopant atoms beneath the surface of semiconductor samples.
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
10:28
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
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Cited by 2 •
2018
Here, we present a protocol to investigate the structure and dynamics of interfacial water at the atomic scale, in terms of submolecular resolution imaging, molecular manipulation, and single-bond vibrational spectroscopy.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 105 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
FAQs
During a scan, the sharp probe moves across the sample surface and records local interactions. In atomic force microscopy, these interactions include forces between the probe and surface. The measurement pattern is converted into a topographic image, so differences in surface position become spatially resolved features.
Its contrast does not depend on light, allowing researchers to examine surface organization through probe-sample interactions. This approach provides information about surface texture and biomolecular arrangement at nanometer-scale resolution. For biological specimens, it therefore complements light-based observations by emphasizing the physical structure of the exposed surface.
Liquid measurements can place the sample in biologically relevant conditions rather than restricting analysis to a dry environment. Under those conditions, the technique can examine cellular or molecular surfaces while retaining information about their texture and organization. This is particularly relevant for interpreting structures in relation to biological function.
Force measurements add mechanical information to the structural image. Interactions between the probe and sample surface can therefore support analysis of how biological material responds mechanically, not only where features are located. Combining surface texture with mechanics helps researchers investigate cell structure, molecular interactions, and links between physical properties and biological function.
A typical workflow places the biological sample within range of a sharp probe, scans the probe across the relevant surface, and records local interactions throughout that scan. The collected measurements are then used to construct a topographic image. When appropriate, measurements may be performed in liquid to provide biologically relevant conditions.
Applications include cell membranes, cytoskeletal features, microbial surfaces, and protein assemblies. These targets represent different levels of biological organization, from cellular boundaries and internal structural elements to microbial and molecular surfaces. Imaging them can reveal how surface architecture and organization relate to broader questions about cell structure and biological function.
The data can support studies of cell structure, molecular interactions, and biological function. Topographic images show the organization and texture of surfaces, while force measurements provide information about mechanical properties. Together, these outcomes allow researchers to connect visible nanoscale architecture with interactions and physical characteristics relevant to biological systems.