Termite Gut Microbiome

The termite gut microbiome is the diverse community of bacteria, archaea, and single-celled eukaryotes living in a termite’s digestive tract, where it supports nutrition and helps termites exploit woody diets. In the hindgut, microbial enzymes break down cellulose and other plant polymers, while fermentation converts released compounds into short-chain fatty acids that termites can absorb; some microbes also use hydrogen and produce methane. Studying this symbiosis links microbial ecology, animal biology, and nutrient cycling, while informing research on lignocellulose degradation, greenhouse-gas production, and potential bioenergy or industrial enzyme applications.

Termite Gut Microbiome - Related Videos

Research

JoVE Journal - Biology

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

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Cited by 18 •

2007

This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

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Cited by 11 •

2010

We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

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Cited by 68 •

2016

We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

Investigating the Microbial Community in the Termite Hindgut - Interview

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2007

Jared Leadbetter explains why the termite-gut microbial community is an excellent system for studying the complex interactions between microbes. The symbiotic relationship existing between the host insect and lignocellulose-degrading gut microbes is explained, as well as the industrial uses of these microbes for degrading plant biomass and generating biofuels.

Research

JoVE Journal - Biology
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A Gut-on-a-Chip Model to Study the Gut Microbiome-Nervous System Axis

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Cited by 10 •

2023

neuroHuMiX is an advanced gut-on-a-chip model to study the interactions of bacterial, epithelial, and neuronal cells under proximal and representative co-culture conditions. This model allows unravelling of the molecular mechanisms underlying the communication between the gut microbiome and the nervous system.

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