Ganglion Cell Recording
Ganglion Cell Recording - Related Videos
13:02
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
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Cited by 26 •
2011
This article provides a description of how to dissect and record from the isolated retinal preparation in mouse. In particular, we describe how to record light responses from a fluorescently labeled ganglion cell population and subsequently identify and analyze its morphology.
11:26
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
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Cited by 8 •
2017
Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.
06:34
Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures
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Cited by 1 •
2011
Time lapse imaging of 3D tissue culture allows studying migratory behavior of individual cells originating from ganglionic eminence in reaction to fractionated protein extract from cerebral cortex.
03:20
Identification of Mouse Retinal Ganglion Cell Subtypes Through Fluorescent Tracing
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2025
Source: Laboissonniere, et. al. Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells. J. Vis. Exp. (2017). This video demonstrates a fluorescent tracing technique to identify retinal ganglion cell (RGC) subtypes in a transgenic mouse retina. The process involves securing the isolated retina in a recording chamber, maintaining cell viability with oxygenated perfusion, and targeting fluorescently labeled RGCs using a pipette with a tracer. By analyzing dendritic morphology and...
05:26
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Cited by 5 •
2011
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
FAQs
Receptive fields indicate which aspects of a sensory stimulus influence a ganglion cell, while firing patterns show how that information appears in its electrical output. By examining these features together, researchers can assess how synaptic inputs are transformed into action potentials and determine how retinal circuits encode visual signals for transmission to the brain.
Extracellular electrodes detect action potentials from ganglion cells without directly measuring the cell interior. Patch-clamp techniques can measure changes in membrane voltage or current, providing information about electrical conditions within the cell. The choice between them depends on whether the study emphasizes firing output or membrane-level responses to controlled sensory stimulation.
Controlled stimulation allows researchers to relate changes in electrical activity to defined sensory inputs rather than to unspecified environmental variation. In retinal preparations, manipulating visual stimulation while monitoring action potentials, membrane voltage, or current helps reveal how ganglion cells respond to particular features and how synaptic signals shape their output.
A typical experiment combines a retinal preparation, an extracellular electrode or patch-clamp setup, and controlled sensory stimulation. The recording system detects action potentials or measures membrane voltage and current while the preparation receives defined visual inputs. Researchers then compare the electrical responses with the stimulation conditions to characterize sensory signaling.
Researchers apply this approach when they need to study sensory processing at the level of retinal circuits and their output neurons. It supports investigations of neural circuit organization, development, and disease. Because recordings reveal how sensory information is transformed into electrical activity, they also help evaluate retinal function in experimental studies.
Recordings provide measurable electrical outcomes, including action potentials and changes in membrane voltage or current. Researchers can compare these responses before and after an intervention, or across different controlled stimulation conditions, to determine whether retinal signaling has been restored or modified. This makes the method useful for assessing approaches intended to influence neural communication.