Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
 

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Article DOI: 10.3791/54103
December 5th, 2016

Chapters

Summary December 5th, 2016

We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter