Tobacco Plant
Tobacco Plant - Related Videos
13:05
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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Cited by 9 •
2014
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
11:06
Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
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Cited by 27 •
2016
Three heat precipitation methods are presented that effectively remove more than 90% of host cell proteins (HCPs) from tobacco extracts prior to any other purification step. The plant HCPs irreversibly aggregate at temperatures above 60 °C.
09:25
Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
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Cited by 7 •
2015
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
10:50
Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
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Cited by 5 •
2014
Plant biomass offers a renewable resource for multiple products, including fuel, feed, food, and a variety of materials. In this paper we investigate the properties of tobacco tree (Nicotiana glauca) and poplar as suitable sources for a biorefinery pipeline.
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Cited by 8 •
2014
This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.
FAQs
Nicotine synthesis is concentrated primarily in the roots, after which the compound is transported to the leaves. This separation links below-ground metabolism with above-ground defense: leaves become the principal site where nicotine can deter herbivores. For plant biology, the arrangement provides a useful example of how specialized metabolites are produced in one tissue and distributed to another.
Photosynthesis supplies chemical energy from light, while specialized metabolic pathways support nicotine synthesis. These processes represent different but connected aspects of tobacco biology: energy conversion supports growth and metabolism, whereas nicotine production contributes to defense. Examining both helps researchers relate general plant physiology to a compound associated specifically with the genus Nicotiana.
Rapid growth, large leaves, and well-characterized genetics make tobacco useful as an experimental plant. Rapid growth can support timely observation, large leaves provide substantial tissue for study, and established genetic information helps researchers connect gene expression with physiological traits. Together, these features allow investigations of plant processes and genetic regulation in a tractable biological system.
Tobacco plants support studies of plant physiology, gene expression, and pathogen interactions. Researchers can use them to examine how plant functions are regulated, how genes are expressed, and how plants respond to pathogens. Because the species also grows rapidly and has large leaves, it offers a practical system for connecting these questions with observable plant traits.
Tobacco plants can be used to produce recombinant proteins, extending their importance beyond studies of natural plant functions. This application uses the plant as a biological production system while retaining its value for investigating gene expression and physiology. Consequently, the same organism can support both fundamental biology and biotechnology research focused on protein production.
The plant connects biological research with agriculture and public health. Agricultural work concerns tobacco production and its consequences, while public-health analysis considers tobacco use and the biological effects of tobacco-derived chemicals. Studying the plant therefore helps link nicotine-related biology and specialized chemistry to broader outcomes without treating the crop solely as a laboratory model.