Tobacco Rattle Virus
Tobacco Rattle Virus - Related Videos
09:25
Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
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Cited by 7 •
2015
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
10:10
Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance
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Cited by 2 •
2022
Here, a protocol is presented for the efficient and accurate screening of tobacco genotypes for Phytophthora nicotianae resistance in seedlings. This is a practical approach for precision breeding, as well as molecular mechanism research.
13:05
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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Cited by 9 •
2014
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Cited by 8 •
2014
This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.
11:10
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
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Cited by 56 •
2014
Formation of protein complexes in vivo can be visualized by bimolecular fluorescence complementation. Interaction partners are fused to complementary parts of fluorescent tags and transiently expressed in tobacco leaves, resulting in a reconstituted fluorescent signal upon close proximity of the two proteins.
FAQs
Stubby-root nematodes introduce TRV into plant roots, after which the virus can move through plant tissues. This progression helps explain why infection may remain associated with localized necrotic lesions in some cases yet produce systemic symptoms and growth abnormalities in others. The pattern of movement connects the infection site with the plant-wide outcome.
These nematodes act as transmission agents: they acquire TRV and introduce it into plant roots. That route makes root exposure central to the early stage of infection and links the host plant to a soil-associated biological pathway. In plant pathology, this relationship helps explain how disease begins before symptoms become visible in aboveground tissues.
Localized effects remain associated with limited regions of infected tissue, such as necrotic lesions, whereas systemic effects extend through the plant and may include broader growth abnormalities. Distinguishing these outcomes helps researchers describe how far infection-related effects have spread and evaluate whether TRV produces a confined response or a more extensive disruption of plant development.
Engineered TRV vectors provide a way to reduce expression of selected plant genes. Researchers use this virus-based approach to examine what changes when a gene becomes less active, connecting gene expression with observable biological traits. The method supports functional studies without relying only on naturally occurring disease symptoms, making TRV useful in molecular biology experiments.
Reducing a targeted gene's expression can help investigators assess its contribution to plant gene function, development, and disease resistance. The same strategy can also support studies of plant interactions with pathogens. These applications turn TRV from a subject of plant pathology into an experimental tool for connecting specific genes with broader biological processes.
Natural TRV infection provides a plant disease context that includes nematode transmission, tissue movement, lesions, and growth abnormalities. Engineered TRV vectors extend that context by enabling targeted gene silencing. Studying both aspects allows researchers to relate infection biology to gene function, development, resistance, and interactions between plants and pathogens.