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Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance
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Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance

Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance

DOI:
10.3791/63054-v

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10:10 min

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April 15, 2022

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, , , , , , ,
1Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute,Chinese Academy of Agricultural Sciences, 2Institute of Rural Development,Zhejiang Academy of Agricultural Sciences, 3Yunnan Academy of Tobacco Agricultural Sciences

Chapters

  • 00:01Introduction
  • 00:17Materials
  • 00:53Planting Tobacco Genotypes for P. nicotianae Resistance Evaluation
  • 02:07Preparation of Oatmeal Agar Medium
  • 03:11Mycelial Cultivation
  • 03:46Preparation of P. nicotianae Zoospore Suspension
  • 04:32Identification of Disease-Resistant Tobacco Varieties
  • 05:36Evaluation of P. nicotianae Infection
  • 06:49Representative Results
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol is presented for the efficient and accurate screening of tobacco genotypes for Phytophthora nicotianae resistance in seedlings. This is a practical approach for precision breeding, as well as molecular mechanism research.

Tags

Keywords: Tobacco GenotypesPhytophthora NicotianaeResistance ScreeningSeedlingsPrecision BreedingMolecular Mechanism ResearchBeinhart1000-1Beinhart1000 BHXiaohuangjin1025 XHJVermiculitePotting SoilHydroponic DevicesHoagland Nutrient SolutionOatmeal Agar MediumMycelial Cultivation

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