Toxicological Assessment
Toxicological Assessment - Related Videos
08:59
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
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Cited by 3 •
2020
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
11:38
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
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Cited by 23 •
2016
The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.
09:33
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Cited by 1 •
2018
This manuscript describes the use of genetically-encoded fluorogenic reporters in an application of live-cell imaging for the examination of xenobiotic-induced oxidative stress. This experimental approach offers unparalleled spatiotemporal resolution, sensitivity, and specificity while avoiding many of the shortcomings of conventional methods used for the detection of toxicological oxidative stress.
09:25
Assessment of Ultrasonic Vocalizations During Drug Self-administration in Rats
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Cited by 17 •
2010
Drug self-administration and ultrasonic vocalizations (USV) are used as behavioral assessments in animal research, but rarely in combination. The purpose of this article is to describe the advantages of recording USVs during drug self-administration procedures to assess affective responses to drug experience.
07:28
A Toxicological and Ecotoxicological Assay Based on Mussel (Mytilus galloprovincialis) Hemocytes Motility
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2024
The article proposes a novel in vitro method for the rapid and sensitive assessment of the toxicity and ecotoxicity of pollutants, based on the motility of Mytilus galloprovincialis hemocytes. The method aims to contribute to the development of more ethical and sensitive toxicological and ecotoxicological exposure tests.
FAQs
Dose provides the basis for relating the amount of a substance encountered to the biological response observed. Interpreting this relationship alongside exposure route and duration helps researchers determine whether changes are associated with a particular exposure pattern. This supports comparisons among substances and contributes to evidence-based evaluations of potential hazards and safe use.
Different endpoints reveal different forms of biological disturbance. Cell viability can indicate damage at the cellular level, while organ function, genetic damage, behavior, and physiology provide information about effects at progressively broader levels. Examining several measurements helps connect exposure with adverse outcomes rather than relying on a single indicator of toxicity.
The same substance may produce different findings depending on how exposure occurs and how long it continues. Toxicological assessment also supports comparisons between susceptible populations, recognizing that biological characteristics can influence responses. Considering these variables prevents researchers from interpreting toxicity as a property of the substance alone and strengthens the biological context of risk evaluation.
A study may combine measurements of cell viability, organ function, genetic damage, behavior, and other physiological changes. The selected measurements should correspond to the biological response being investigated and the exposure conditions under consideration. Comparing these results with the relevant dose, route, and duration allows researchers to characterize how exposure is linked to adverse effects.
Its applications extend across drug development, environmental monitoring, risk evaluation, and investigations of disease mechanisms. In drug development, findings can inform judgments about potential harmful effects. Environmental studies use the approach to examine effects on organisms and ecosystems, while disease research uses exposure-related biological changes to explore how adverse processes may arise.
Results provide evidence for linking a specific exposure pattern with biological or adverse outcomes. Researchers can use that evidence to characterize hazards, compare responses among populations, and support risk evaluation. These conclusions help inform safeguards intended to protect human health and ecosystems, particularly when decisions must account for dose, exposure route, duration, and measured biological effects.