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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
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Biology
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JoVE Journal Biology
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)

DOI:
10.3791/53987-v

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11:38 min

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May 10, 2016

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, , , , , , ,
1Biological System Research (BSR),Philip Morris International R&D

Chapters

  • 00:05Title
  • 01:03Real Time Cell Analyzer (RTCA)-based Dose Range Finding (DRF)
  • 04:26Preparation for Measuring Toxicological Effects by High-content Screening (HCS)
  • 06:27Cytotoxicity Assay
  • 09:19Results: Representative Results for 1-Aminonaphtalene
  • 10:26Conclusion

Summary

Automatic Translation

Automatic Translation

The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.

Tags

Keywords: High Content ScreeningToxicological EffectsHarmful And Potentially Harmful Constituents (HPHC)Impedance-based Real-time Cell AnalyzerCytotoxicityGenotoxicityCellular Stress ResponseNHBE CellsStaurosporineDose-responseToxicity Profiling

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