Transducer Scan

Transducer scanning is an imaging approach that uses a transducer to generate and detect waves, converting returned energy into signals that represent structures within a biological sample or organism. In ultrasound-based scanning, the transducer emits high-frequency sound waves into tissue; differences in acoustic properties at tissue boundaries produce echoes, which the system processes into a two-dimensional or three-dimensional image. This noninvasive method supports examination of organs, developing embryos, blood flow, and other soft tissues, while allowing repeated observation without exposing specimens to ionizing radiation. Its biological applications include anatomical assessment, developmental studies, and clinical research.

Transducer Scan - Related Videos

Education

JoVE Science Education - Engineering

Pressure Transducer: Calibration Using a Pitot-static Tube

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2023

Source: Shreyas Narsipur, Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC Fluid pressure is an important flow characteristic that is required to determine the aerodynamics of a system. One of the oldest and still existing pressure measurement systems is the manometer due to its accuracy and simplicity of operation. The manometer is generally a U-shaped glass tube that is partially filled with liquid, as shown in Figure 1. The U-tube manometer requires no...

Leaky Scanning

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2020

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

Research

JoVE Journal - Neuroscience

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

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Cited by 44 •

2012

In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.

Research

JoVE Journal - Immunology and Infection
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Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

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Cited by 11 •

2010

Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.

Education

JoVE Core - Pharmacology
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Transducer Mechanism: Nuclear Receptors

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2023

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors. About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

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