Transgenic Root Tissue
Transgenic Root Tissue - Related Videos
09:41
Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
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Cited by 9 •
2011
We present a method for chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury. The approach can be used to identify specific transcription factor binding sites and epigenetic modification of histone and DNA important for the regeneration of injured axons in both the peripheral and central nervous system.
09:27
High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
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Cited by 4 •
2016
Crystalline cellulose is an important constituent of the plant cell wall. However, its quantification at a cellular resolution is technically challenging. Here, we report the use of polarized light technology and root cross sections to obtain information of cell wall composition at a spatiotemporal resolution.
02:16
Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
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2025
This video demonstrates the generation of neural progenitor cells from non-transgenic and transgenic human pluripotent stem cells (hPSCs). hPSCs are differentiated into neural progenitor cells using a neuronal differentiation medium. While differentiation is induced in non-transgenic cells using small molecules, the transgenic hPSCs are differentiated by adding an antibiotic that induces the promoter for a neuronal differentiation-specific transcription factor.
12:17
Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
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Cited by 4 •
2017
Full-root aortic valve replacement by stentless aortic xenograft is a viable option in patients with small aortic roots. We describe, a technique for the full-root implantation of stentless aortic xenografts, with emphasis on the management of the proximal suture line and coronary anastomoses, and discuss its limitations and alternative options.
13:54
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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Cited by 43 •
2012
This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.
FAQs
Agrobacterium rhizogenes uses its Ri plasmid as the source of transferable T-DNA. The bacterium transfers this DNA into plant cells, where the inserted sequence becomes integrated into the plant genome. Once retained and expressed, the introduced genetic material can produce transformed root tissue, often with the rapidly growing hairy-root phenotype described for this system.
Integration makes the introduced DNA part of the plant genome rather than a transient component of the cells. This genomic placement supports continued carriage and expression of the introduced gene in the resulting root tissue. Consequently, researchers can examine gene function or connect genetic changes with root development and specialized metabolite production.
Their rapid growth makes hairy-root cultures a practical, focused model for investigating processes centered on roots. Researchers can use them to examine root development, test gene-function questions, and study plant-microbe interactions without treating root biology as only one component of a whole-plant system. The same cultures can also support specialized-metabolite analysis.
Because the tissue carries and expresses an introduced gene, researchers can relate that genetic activity to metabolic pathways in roots. Analysis of the resulting tissue can focus on specialized metabolites, helping reveal how root-associated genetic processes relate to compound production. This makes the system useful for both mechanistic biology and biotechnology-oriented studies.
The process begins with plant cells encountering Agrobacterium rhizogenes, whose Ri plasmid supplies transferable T-DNA. That DNA enters the plant cells and becomes integrated into their genome. The transformed cells then give rise to root tissue that carries and expresses the introduced gene, with rapidly growing hairy roots commonly associated with the transformation outcome.
It is especially useful when the research question centers on roots, root development, or root-associated metabolism. The focused tissue supports gene-function studies and investigations of plant-microbe interactions while allowing specialized metabolites to be produced and analyzed. Researchers can therefore examine root-specific biology and compound-related outcomes in a system aligned with those questions.
Metabolite analysis can show how genetically transformed root tissue produces specialized compounds and can help researchers investigate the metabolic pathways involved. These results support studies that link gene activity with biochemical output. Beyond basic biology, the ability to produce and analyze valuable compounds gives the cultures relevance to biotechnology, agriculture, and pharmaceutical research.
The transformation system links a bacterial process with a plant-root research model: Agrobacterium rhizogenes initiates genetic transformation through Ri-plasmid T-DNA transfer, while the resulting root tissue provides material for biological study. Researchers can use this context to investigate plant-microbe interactions alongside root development and gene function, making the model relevant to multiple areas of biology.