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JoVE Journal
Developmental Biology

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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
 

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Article DOI: 10.3791/60927-v
March 4th, 2020

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Summary March 4th, 2020

Here, we describe the immunoprecipitation of ribosomes and associated RNA from select populations of adult male mouse germ cells using the RiboTag system. Strategic breeding and careful immunoprecipitation result in clean, reproducible results that inform on the germ cell translatome and provide insight into the mechanisms of mutant phenotypes.

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