Glioblastoma Xenoline
Glioblastoma Xenoline - Related Videos
10:52
Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme
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Cited by 42 •
2012
We describe an integrated method for the precise, stereotactic implantation of human glioblastoma multiforme cells into the brains of nude mice and subsequent serial in vivo imaging to monitor growth and response to treatment of the resultant xenografts.
07:52
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
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Cited by 5 •
2018
Orthotopic intracranial injection of tumor cells has been used in cancer research to study brain tumor biology, progression, evolution, and therapeutic response. Here we present fluorescence molecular tomography of tumor xenografts, which provides real-time intravital imaging and quantification of a tumor mass in preclinical glioblastoma models.
03:09
Fluorescence Molecular Tomography: An Imaging Technique for In Vivo Imaging of Fluorescent Protein-tagged Glioblastoma Xenografts in Mouse Model
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2025
In this video, we describe fluorescence molecular tomography or FMT for in vivo imaging of fluorescent protein-tagged glioblastoma xenografts in mouse models. The technique is useful for elucidating the effects of anti-tumor therapeutics at the molecular level.
02:36
Generating a Glioblastoma Rat Model
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2025
Source: Bolcaen, J., et. al., PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator. J. Vis. Exp. (2017)This video demonstrates the procedure for establishing a glioblastoma rat model by stereotactically injecting brain tumor-causing glioblastoma cells into the prefrontal cortex.
09:43
Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation
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Cited by 10 •
2014
Malignant gliomas constitute a heterogeneous group of highly infiltrative glial neoplasms with distinct clinical and molecular features. Primary orthotopic xenografts recapitulate the histopathological and molecular features of malignant glioma subtypes in preclinical animal models.
FAQs
Orthotopic transplantation places human glioblastoma cells or tumor tissue into the brain, allowing the tumor to interact with surrounding brain tissue in a living host. This setting supports investigation of growth and invasion in conditions that more closely reflect the disease location than a model established outside the brain, making it useful for studying tumor behavior.
An immunodeficient mouse provides the nonhuman host in which implanted human glioblastoma material can grow as an experimental tumor. Using this host creates a controlled living system for examining tumor biology and treatment responses. The model therefore connects observations from human cancer material with behavior observed after implantation in an organism.
Because a xenoline can be established from human glioblastoma cells or tumor tissue, it may retain characteristics of the source tumor. Researchers can use this feature to compare models derived from different patient tumors and examine whether their growth, invasion, biology, or treatment responses differ, supporting more representative preclinical investigation.
Establishment begins with human glioblastoma cells or tumor tissue and a suitable nonhuman host, typically an immunodeficient mouse. The material may be transplanted orthotopically into the brain, where it can develop within living tissue. This combination supplies the tumor source, host environment, and controlled setting needed for subsequent cancer research.
Researchers can examine glioblastoma growth, invasion, treatment resistance, and broader tumor biology in a living system. These models allow those processes to be studied after human cancer material has been introduced into a host, helping investigators explore how tumors behave and compare biological features across different experimental xenolines.
A xenoline can serve as a preclinical platform for evaluating candidate drugs and therapeutic strategies before clinical testing. Researchers can assess how implanted human glioblastoma responds while also considering tumor growth, invasion, and treatment resistance. Comparing xenolines from different source tumors may further reveal whether a strategy performs similarly across tumor backgrounds.