Human Esophageal Fibroblasts
Human Esophageal Fibroblasts - Related Videos
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
12:16
Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
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Cited by 3 •
2015
This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
10:15
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
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Cited by 12 •
2017
Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.
03:32
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
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2025
The video demonstrates the generation of neurons from adult human dermal fibroblasts (aHDFs). The aHDFs are reprogrammed using a lentiviral vector carrying genes for neuronal transcription factors and short hairpin RNAs (shRNAs). The shRNAs inhibit the expression of a neuronal transcriptional repressor, and the neuronal transcription factors activate genes to differentiate aHDFs into neurons. These reprogrammed aHDFs are cultured in an early and a late neuronal conversion medium to generate...
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These cells adjust their activity in response to both physical forces and biochemical signals in the esophageal environment. Such cues can influence extracellular-matrix production and remodeling, as well as communication with neighboring cells. Studying these responses helps researchers determine how stromal conditions may support tissue repair or create conditions that affect esophageal tumor development.
Matrix remodeling changes the structural environment surrounding cells and can alter how neighboring epithelial, immune, and tumor cells interact with the tissue. Human esophageal fibroblasts are therefore useful for examining how changes in matrix proteins and their organization contribute to stromal behavior. This information can clarify mechanisms linked to tumor invasion and altered treatment responses.
Comparing normal fibroblasts with cancer-associated fibroblasts can reveal differences in cell signaling, matrix remodeling, and tumor-supportive behavior. The comparison focuses on how fibroblast states influence the surrounding microenvironment rather than treating all stromal cells as equivalent. These distinctions may identify processes associated with tumor initiation, invasion, or response to treatment.
Researchers can use these cells as a stromal model to examine how connective-tissue behavior influences esophageal cancer. Experiments may evaluate matrix production and remodeling, responses to environmental cues, and communication with epithelial or immune cells. Together, these observations show how the non-tumor cellular environment may affect tumor development and behavior.
The model supports investigation of communication through secreted factors and direct interactions with neighboring epithelial and immune cells. Researchers can ask how fibroblast-derived signals or physical contact change the behavior of surrounding cells, and how those cells in turn influence fibroblasts. This approach helps connect stromal activity with tissue repair and cancer-associated changes.
Analyses of human esophageal fibroblasts can show whether stromal characteristics are associated with different treatment responses. Comparing normal and cancer-associated cells may reveal signaling or matrix-remodeling features that support tumor persistence or alter how cancer cells respond. Such findings can guide investigation of mechanisms and potential therapeutic targets within the esophageal tumor microenvironment.