Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Nicola H. Green; Bernard M. Corfe; Jonathan P. Bury; Sheila MacNeil

doi:10.3791/52693

Journal

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Bioengineering

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Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

10,437 Views

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12:16 min

•

May 18, 2015

DOI:

10.3791/52693-v

DOI:

10.3791/52693-v

•

12:16 min

May 18, 2015

•

3 Views

Nicola H. Green¹, Bernard M. Corfe², Jonathan P. Bury³, Sheila MacNeil¹

¹Department of Materials Science and Engineering,University of Sheffield, ²Department of Oncology and Insigneo Institute for in silico Medicine,University of Sheffield, ³Department of Histopathology,Sheffield Teaching Hospitals NHS Foundation Trust

Summary

This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

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