This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.
Green, N. H., Corfe, B. M., Bury, J. P., MacNeil, S. Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model. J. Vis. Exp. (99), e52693, doi:10.3791/52693 (2015).