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Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
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Chapters
- 00:05Title
- 02:05Human Esophageal Epithelial Cell and Fibroblast Isolation
- 04:08De-cellularized Pig Esophageal Scaffold Preparation
- 06:27Human Esophageal Mucosa Model Preparation
- 09:44Results: Representative 3D Tissue-engineered Human Esophageal Mucosa
- 11:26Conclusion
This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.
Tags
Esophageal MucosaEsophageal AdenocarcinomaBarrett’s MetaplasiaEsophageal FibroblastsEsophageal Epithelial CellsAcellular Esophageal ScaffoldImmunohistochemical CharacterizationCK4CK14Ki67InvolucrinBiologically Relevant Experimental ModelPharmacological AgentsEnvironmental FactorsAlcoholToxinsHigh TemperatureGastro-esophageal RefluxExtended CultureEsophageal Tumor Cell LinesBarrett’s Cell LinesTumor InvasionDrug Responsiveness
