Microcarrier Fabrication
Microcarrier Fabrication - Related Videos
04:13
Bioscaffold Fabrication by Electrospraying: A Technique to Generate Microcarrier Scaffolds Derived from Extracellular Matrix of Decellularized Tissue
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2023
This video demonstrates the procedure of extracellular matrix-derived microcarrier fabrication by electrospraying. These microcarriers simulate the native tissue-specific microenvironment and are stable in long-term 3D in vitro cell culture models.
11:19
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
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Cited by 26 •
2017
The tissue-specific extracellular matrix (ECM) is a key mediator of cell function. This article describes methods for synthesizing pure ECM-derived foams and microcarriers that are stable in culture without the need for chemical crosslinking for applications in advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
07:36
Fabricating Nanogaps by Nanoskiving
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Cited by 3 •
2013
The fabrication of electrically addressable, high-aspect-ratio (> 1000:1) metal nanowires separated by gaps of single nanometers using either sacrificial layers of aluminum and silver or self-assembled monolayers as templates is described. These nanogap structures are fabricated without a clean room or any photo- or electron-beam lithographic processes by a form of edge lithography known as nanoskiving.
08:29
Fabrication of Amperometric Electrodes
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Cited by 8 •
2009
This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.
05:40
Fabricating Cotton Analytical Devices
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2016
To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.
FAQs
Particle size affects the available area for cell attachment and the behavior of carriers in stirred or suspension cultures. Porosity can provide additional surface features and influence how cells interact with the particle. Controlling both properties helps balance cell expansion, nutrient access, and recovery, making these variables important when designing microcarriers for reproducible culture systems.
Surface chemistry determines how effectively anchorage-dependent cells attach to the particle. A suitable surface can support initial adhesion and subsequent proliferation, whereas an unsuitable one may reduce the usable carrier surface. In cancer research, this control helps researchers maintain tumor or stromal cell populations during expansion and create cultures that better reflect cell-matrix interactions.
Mechanical properties influence how particles maintain their structure during culture and how cells experience the surrounding matrix. They must be considered alongside size, porosity, and surface chemistry because these features collectively shape attachment, proliferation, and recovery. Adjusting this combination can help construct three-dimensional cancer models with physical environments relevant to tumor biology.
A general workflow begins by selecting a polymeric or hydrogel-based material, forming it into particles, and stabilizing the resulting structures. Fabrication then focuses on controlling size, porosity, surface chemistry, and mechanical properties so the particles provide a suitable adhesive environment. The finished carriers can be introduced into stirred or suspension cultures for cell expansion.
Microcarrier-based systems are useful when researchers need scalable culture of anchorage-dependent tumor or stromal cells or want a three-dimensional setting. Unlike flat cultures, they can provide cell-cell and cell-matrix interactions within a more structured environment. This makes them relevant for tumor modeling and for studies examining how physical microenvironments influence cancer progression or treatment response.
These cultures can support tumor modeling, drug screening, and investigation of treatment response in a three-dimensional context. They also allow researchers to examine how the physical microenvironment affects cancer progression while expanding relevant tumor or stromal cells. The resulting platform connects controlled particle properties with biological outcomes such as proliferation and recovery.