Partition Collection
Partition Collection - Related Videos
01:14
Extraction: Partition and Distribution Coefficients
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2024
The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium. For extracting a solute from an aqueous phase into an organic...
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
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Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
11:11
Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
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Cited by 5 •
2018
Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.
11:50
Metal-silicate Partitioning at High Pressure and Temperature: Experimental Methods and a Protocol to Suppress Highly Siderophile Element Inclusions
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Cited by 5 •
2015
We present a procedure to determine the metal-silicate partitioning of siderophile elements, emphasizing techniques that suppress the formation of metal inclusions in experiments for the noble metals. The results of these experiments are used to demonstrate the effect of core-formation on the highly siderophile element composition of the mantle.
08:23
Seawater Sampling and Collection
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Cited by 13 •
2009
This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.
FAQs
Maintaining the identity of each droplet, well, or other compartment allows researchers to connect a recovered fraction with its original sample context. This is important when comparing tumor cells, nucleic acids, or treatment responses across separated compartments. If identities are not retained when needed, compartment-specific differences may be obscured during downstream analysis.
Controlled transfer conditions help preserve the contents of each compartment while material is recovered or pooled. This supports reliable separation between sample fractions and reduces uncertainty about which material enters a downstream assay. Consistent handling is especially relevant when researchers need well-defined fractions for sequencing, imaging, or analysis of treatment responses.
Pooling combines selected recovered material for downstream analysis, whereas keeping partitions separate preserves information about individual compartments. The choice depends on whether the study emphasizes overall sample composition or compartment-specific differences. In cancer research, separate handling can support studies of cellular heterogeneity and rare molecular signals, while pooling can provide a combined sample fraction.
A workflow begins with physically separated sample compartments such as droplets or wells. Researchers then transfer or pool the compartments under controlled conditions, while preserving their contents and retaining individual identities when required. The recovered material becomes a defined input for downstream procedures, including sequencing, imaging, or other assays that depend on separated sample fractions.
The approach is useful when cancer studies need to examine differences among tumor cells, nucleic acids, or treatment responses within a divided sample. Recovering defined compartments can help researchers investigate cellular heterogeneity and identify rare molecular signals. It also provides organized sample fractions for later sequencing, imaging, or related analytical workflows.
Reliable collection improves the consistency of assays that depend on well-defined sample fractions. Preserving compartment contents and, when necessary, their identities helps ensure that sequencing or imaging receives the intended material. In cancer research, this can support clearer interpretation of heterogeneous tumor samples and treatment-related differences by linking results to the appropriate recovered fraction.