Helium Lamp
Helium Lamp - Related Videos
06:57
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
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Cited by 10 •
2015
Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
05:03
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
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Cited by 11 •
2018
This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.
08:03
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
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Cited by 8 •
2018
This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.
07:47
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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Cited by 5 •
2020
We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.
01:17
Balmer Series - Instructor Prep
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of the LaboratoryExpand Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Place a power base for gas discharge lamps in a central area. Pick up the hydrogen lamp and clean it with...
FAQs
An applied electric current causes collisions that transfer energy to helium atoms in the low-pressure gas. These collisions promote atoms to higher electronic states. As the atoms return to lower states, they emit photons with specific wavelengths rather than a continuous range, producing the separated lines observed in the helium spectrum.
Each observed line corresponds to a change between allowed electronic energy states in helium. The resulting wavelengths therefore reflect the atom’s electronic structure. Studying these patterns connects visible spectral observations with quantized energy levels, showing how atomic theory explains the specific light released by an excited element.
The lamp uses helium at low pressure so an electric current can pass through the gas and produce collisions that excite its atoms. This operating condition supports the formation of the characteristic emission pattern. The emitted lines can then be examined as a practical signal of helium’s energy-state transitions.
The lamp demonstrates that matter can absorb electrical energy and release it as light at discrete wavelengths. This sequence links energy transfer, atomic excitation, and photon emission in one observable system. For chemistry students, it provides a concrete way to connect light-matter interactions with quantized electronic behavior.
Because helium produces characteristic emission lines at discrete wavelengths, its spectrum can serve as a reference when calibrating optical instruments. A researcher compares the observed lines with their expected wavelength positions and uses that relationship to assess or adjust the instrument’s wavelength measurement. This supports reliable spectroscopic analysis.
The line pattern provides evidence of helium’s electronic structure and offers identifiable spectral features for optical measurements. In chemistry, those features support spectroscopy, demonstrations of quantized energy levels, and analytical measurement. The lamp is especially useful when a controlled source of characteristic emission is needed to examine how an instrument or spectrum represents light.