Autonomy

Autonomy is the capacity and ethical right of individuals to make informed, voluntary decisions about their own lives and care. In clinical practice, it is supported when patients receive understandable information, have decision-making capacity, and can accept or refuse interventions without coercion. Respecting autonomy underlies informed consent, shared decision-making, advance care planning, and confidentiality, while clinicians help patients clarify values and understand risks, benefits, and alternatives. Applying this principle promotes patient-centered care and trust, although it may require careful communication and ethical judgment when capacity is impaired, preferences conflict with professional recommendations, or decisions affect others.

Autonomy - Related Videos

Research

JoVE Journal - Developmental Biology

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

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Cited by 12 •

2016

This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

Research

JoVE Journal - Neuroscience
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Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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Cited by 20 •

2013

In this article we describe the full experimental procedure to reconstruct, with high resolution, the fine brain anatomy of fluorescently labeled mouse brains. The described protocol includes sample preparation and clearing, specimen mounting for imaging, data post-processing and multi-scale visualization.

Research

JoVE Journal - Biology
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Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

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Cited by 20 •

2010

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

Generating Chimeric Zebrafish Embryos by Transplantation

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Cited by 61 •

2009

A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

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