Cytokine-containing Patch
Cytokine-containing Patch - Related Videos
12:58
Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
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Cited by 2 •
2017
Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.
09:43
Patch Clamp Electrophysiology
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2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
01:02
Long-patch Base Excision Repair
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2020
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are: Lesion type: Depending on the type of base damage, a specific DNA glycosylase - mono or bifunctional, is recruited to the damaged site. While the sequential action of a monofunctional glycosylase favors long patch repair...
02:57
Assessing Antiviral Cytokine Production in Response to Viral Infection
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2026
Source: Zhang, J., et al. Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production. J. Vis. Exp. (2014)This video demonstrates the process of infecting murine fibroblast cells with herpesvirus and monitoring the resulting antiviral cytokine release and gene expression. It outlines key steps, including viral infection, media collection for cytokine quantification, and cell harvesting for gene expression analysis.
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
FAQs
Its main experimental value is the ability to establish a signaling environment at a defined location while releasing cytokines over a controlled period. This spatial and temporal organization helps investigators examine how changing cytokine exposure influences cell survival, proliferation, migration, and differentiation during tissue formation, rather than treating signaling as uniform throughout a system.
Retention keeps cytokines within or on the biomaterial until release at the intended site. This helps concentrate signaling where it is needed and can limit exposure to surrounding tissues. The resulting localization is important when researchers want to connect a developmental response with a particular signaling region instead of with widespread cytokine distribution.
Localized delivery allows developmental biology experiments to relate cellular behavior to a defined source of cytokine signaling. Because these signals can influence survival, proliferation, migration, and differentiation, a patch can help reveal how their position and timing contribute to tissue formation or repair. This provides a more spatially organized experimental context than unrestricted exposure.
A basic setup combines a biomaterial patch with cytokines retained within or on its structure and places that construct at a target site. The experimental design then considers where the patch is positioned and how cytokine release is controlled. These elements create a defined signaling environment for observing responses in nearby cells or tissues.
Researchers may use the platform when engineered tissues or organoids require a localized, defined cytokine environment. By positioning signaling near the developing model, investigators can study effects on cell survival, proliferation, migration, or differentiation while limiting exposure beyond the intended region. This supports experiments focused on how patterned signaling contributes to tissue development or repair.
The platform can support evaluation of cellular and tissue responses linked to cytokine signaling, including changes in survival, proliferation, migration, and differentiation. In developmental biology, these outcomes help assess how spatial and temporal signaling patterns shape tissue formation. Similar observations can inform engineered tissue models, organoid research, and regenerative strategies that require localized communication between cells.