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The Complete and Updated “Rotifer Polyculture Method” for Rearing First Feeding Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
The Complete and Updated “Rotifer Polyculture Method” for Rearing First Feeding Zebrafish

The Complete and Updated “Rotifer Polyculture Method” for Rearing First Feeding Zebrafish

DOI:
10.3791/53629-v

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08:52 min

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January 17, 2016

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, , , , , , , ,
1Aquatic Resources Program,Boston Children’s Hospital, 2UQ Biological Resources,University of Queensland, 3Reed Mariculture, 4Aquatics Lab Services

Chapters

  • 00:05Title
  • 00:44Rotifer Culture Maintenance
  • 02:47Rotifer Harvest
  • 05:08Polyculture Setup and Inoculation
  • 06:29Polyculture Phase
  • 07:24Results: Successful Implementation of Rotifer Polyculture Method
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

Larval zebrafish are adapted to feed on zooplankton. It is possible to capitalize on this natural feature in the laboratory by growing first feeding fish together in the same system with live saltwater rotifers. This "polyculture" strategy promotes high growth and survival with minimal labor and disturbance to the larvae.

Tags

Rotifer Polyculture MethodRearingFirst Feeding ZebrafishRapid GrowthMaximum SurvivalRotifer Culture MaintenanceFeed-out Culture VesselFCVWater DosingAquarium SaltsSalinity MeasurementCulture VesselCVSample CollectionCulture Quality AssessmentLugol’s Iodine Solution

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