High-speed Microscopy System
High-speed Microscopy System - Related Videos
07:53
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
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Cited by 4 •
2018
Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.
11:15
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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Cited by 114 •
2016
This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.
04:56
High-Speed Time-Lapse Atomic Force Microscopy to Capture Nucleosome Dynamics
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2025
In this video, we demonstrate high-speed time-lapse atomic force microscopy, AFM, imaging techniques to study the dynamics of nucleosome complexes. As the AFM tip scans across the surface of the nucleosome, it detects alterations in the nucleosome height and records the DNA unwrapping over time.
09:45
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
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Cited by 19 •
2019
This protocol is a guide for implementing interference reflection microscopy on a standard fluorescence microscope for label-free, high-contrast, high-speed imaging of microtubules using in vitro surfaces assays.
05:32
High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia
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Cited by 2 •
2022
High-speed video microscopy analysis is a relatively easy-to-perform, fast, cost-effective, and, in experienced hands, a considerably reliable tool for first-line diagnostics of primary ciliary dyskinesia, which should be available in every center involved in diagnostics and the treatment of severe lung diseases.
FAQs
Temporal resolution determines whether a transient event appears as a sequence of measurable changes or as an apparent jump between states. In a High-speed Microscopy System, rapid frame capture is paired with illumination and optics that limit motion blur while retaining spatial detail. This balance lets engineers distinguish evolving deformation, fluid movement, or process changes rather than infer them from end points.
The fast image sensor records successive frames, controlled illumination supports image capture during rapid changes, and high-speed optics preserve spatial information as events unfold. These components must function together because increasing capture speed alone does not ensure useful measurements. Their combined performance determines whether the recorded sequence retains both the timing and the structural detail needed for engineering analysis.
Synchronized acquisition coordinates image recording with the event being observed, allowing successive frames to represent the correct progression of motion or change. Without coordinated timing, the sequence may not accurately associate images with the physical process. In engineering studies, synchronization strengthens analysis of fluid motion, mechanical deformation, microfabrication, and dynamic material responses by making their evolution easier to compare.
A practical workflow combines the high-speed sensor, controlled illumination, optical arrangement, and synchronized acquisition before recording the event of interest. The resulting image sequence is then examined for changes over time and space. This workflow converts rapid behavior into quantitative observations that can be used to identify failure mechanisms, transport phenomena, or variability in an engineered process.
Applications include observing fluid motion, measuring mechanical deformation, examining microfabrication processes, and studying dynamic material responses. These measurements are useful when important behavior occurs too quickly for conventional observation to resolve directly. By revealing how a device, material, or process changes during operation, the system supports engineering design, testing, and optimization.
Quantitative image sequences show how structures or patterns change across successive moments, helping engineers connect observed motion with process behavior. They can reveal failure mechanisms, transport phenomena, and process variability that may remain hidden in static images or endpoint measurements. This evidence supports comparison among devices or manufacturing conditions and guides improvements to design and processing methods.