Rhizosphere Bacteria

Rhizosphere bacteria are microorganisms that live in the thin layer of soil influenced by plant roots, where they help shape nutrient cycling, plant health, and soil function. Root exudates, including sugars, amino acids, and organic acids, provide carbon that supports bacterial growth, while bacterial enzymes and metabolites influence nutrient availability and interactions with other organisms. These communities can promote plant growth through processes such as nitrogen fixation, phosphate solubilization, and production of compounds that regulate root development or suppress pathogens. Studying rhizosphere bacteria supports sustainable agriculture, soil restoration, and research into how plant–microbe interactions affect environmental resilience.

Rhizosphere Bacteria - Related Videos

Research

JoVE Journal - Environment

A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

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Cited by 5 •

2021

We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging.

An Approach to Constructing Multispecies Biofilm Communities from Rhizosphere Soil

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Cited by 2 •

2024

A rapid and standardized procedure for establishing synergistic multispecies biofilm communities from various rhizosphere soils is presented here. It is a unique protocol designed to probe and simulate the complex rhizosphere soil microbiota.

Education

JoVE Science Education - Environmental Sciences

Culturing and Enumerating Bacteria from Soil Samples

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Authors: Bradley Schmitz and Luisa Ikner Surface soils are a heterogeneous mixture of inorganic and organic particles that combine together to form secondary aggregates. Within and between the aggregates are voids or pores that visually contain both air and water. These conditions create an ideal ecosystem for bacteria, so all soils contain vast populations of bacteria, usually over 1 million...

Research

JoVE Journal - Immunology and Infection
Free Sample

Detection of Bacteria Using Fluorogenic DNAzymes

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Cited by 17 •

2012

We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental...

Isolation of Bacteria from the Mosquito Midgut

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2026

Source: Liu, X., et al. A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes. J. Vis. Exp. (2020)This video demonstrates the dissection of the mosquito midgut to isolate gut bacteria. The midgut is extracted from the digestive tract, homogenized to release the bacteria, and plated on nutrient agar to culture bacterial colonies.

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