In Situ Sensors
In Situ Sensors - Related Videos
08:20
In Situ Soil Moisture Sensors in Undisturbed Soils
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Cited by 7 •
2022
The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.
09:14
Glovebox and Impurity Sensors
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert atmosphere. For manipulations under inert atmospheres, chemists can choose between Schlenk or high-vacuum techniques and a glovebox. Schlenk and...
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
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Cited by 6 •
2013
We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
10:23
Glutamine Flux Imaging Using Genetically Encoded Sensors
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Cited by 7 •
2014
This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
FAQs
The sensing element responds to a measured environmental variable, such as temperature, pressure, dissolved oxygen, pH, or contaminant concentration. A transducer then converts that response into an electrical signal. Recording instruments can preserve these readings, while transmission systems support observation over time. This signal pathway lets one device represent changing local conditions.
Their measurements occur within the environment being studied, so the readings preserve local conditions at the measurement site. Laboratory sampling can separate observation from those conditions, whereas direct deployment supports repeated or continuous observations over time. This distinction matters when environmental variables change, because time-resolved local data can strengthen models and help detect hazards earlier.
Different sensing elements or transducers can support different kinds of observations. Physical variables include temperature and pressure; chemical measurements include pH, dissolved oxygen, and contaminant concentration; biological conditions can also be monitored. Because the output becomes an electrical signal, the measurement system can feed instruments that record data or systems that transmit it.
Deployment can occur in water, soil, air, or ecological monitoring systems, depending on the environmental condition under study. Placing the device in the relevant setting allows measurements to reflect that setting rather than a removed sample. Across these contexts, recorded observations can follow environmental change and provide evidence about pollution or ecosystem health.
A basic monitoring workflow places the sensor in the target environment, connects its output to an instrument that records readings, and, where available, uses transmission to carry measurements over time. The resulting time series can show environmental change, pollution, or ecosystem-health conditions. Continuous records also support earlier hazard detection and provide inputs for stronger environmental models.
Environmental managers can use their high-resolution observations to recognize changing conditions, identify possible pollution, and assess ecosystem health. The value comes from combining local measurements with continuity over time, rather than relying only on isolated observations. These data can improve models and support more responsive management when hazards or other environmental changes emerge.