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A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging
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A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

Article DOI: 10.3791/62227-v • 06:29 min • February 15th, 2021
February 15th, 2021 •
1, 1, 2
1Department of Plant and Soil Sciences, University of Delaware, 2National Synchrotron Radiation Lightsource-II, Brookhaven National Laboratory

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Summary
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We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging.

Tags

Preserve Wetland Roots Rhizospheres Elemental Imaging Method Prepare Aquatic Plant Distribution Speciation Elements Of Interest Quick Affordable Accurate Nutrients Contaminants Sampling Slam Freezing Equipment Liquid Nitrogen Copper Blocks Spacer PPE Potted Plant Shovel Wet Soil
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