Antibiotic Microbiota Interaction
Antibiotic Microbiota Interaction - Related Videos
01:21
Microbiota Modulation by Antibiotics
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2026
Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...
08:33
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
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Cited by 24 •
2018
Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.
04:09
An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions
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2025
In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue viability.
00:06
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
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Cited by 10 •
2019
Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
09:31
Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
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Cited by 10 •
2021
This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.
FAQs
Antibiotic exposure creates a selective pressure within microbial communities. Susceptible organisms decline, while less-sensitive organisms persist, allowing community composition to shift even when treatment targets a particular infection. These changes may also affect microbial functions and resistance patterns, helping explain why antibiotic effects can extend beyond the organisms directly responsible for disease.
The microbiota can affect antibiotic activity, metabolism, and availability, making the interaction reciprocal rather than one-directional. In practical terms, microbial communities may influence how much active drug remains accessible and how its effects are expressed. Considering this relationship helps connect changes in microbial ecology with differences in antimicrobial treatment consequences.
Antibiotic-associated dysbiosis may alter colonization resistance, meaning the microbiota’s ability to limit establishment by other microbes. Changes in community composition and microbial function can also modify host immune responses. Together, these effects connect antimicrobial treatment with infection-related outcomes and immunological changes, rather than treating drug action as separate from the surrounding microbial ecosystem.
Because antibiotics suppress susceptible organisms while allowing less-sensitive organisms to persist, treatment can change the distribution of resistance patterns within a community. The result depends on which organisms are affected and which remain. Examining these shifts is important for understanding how antimicrobial exposure changes microbial communities and their future response characteristics.
A useful evaluation considers several linked outcomes: microbial community composition, microbial functions, resistance patterns, colonization resistance, and host immune responses. Researchers can also examine how the microbiota influences antibiotic activity, metabolism, and availability. Assessing these dimensions together provides a broader view of treatment effects than measuring suppression of a single microorganism alone.
This topic supports more precise antibiotic use by connecting antimicrobial treatment with beneficial microbes, infection-related microbial changes, and host immunity. In immunology and infection research, the interaction helps explain treatment-associated dysbiosis and altered immune responses. It also guides efforts to preserve beneficial microbial communities, reduce collateral effects, and improve infectious-disease outcomes.