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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

DOI:
10.3791/58481-v

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08:33 min

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November 17, 2018

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, ,
1The Peter Gorer Department of Immunobiology,King’s College London, 2The Francis Crick Institute

Chapters

  • 00:04Title
  • 00:39Administration of Antibiotics
  • 02:29Collection of Fecal Samples from Stool, Ileum Content and Ileum Wall
  • 05:16Quantification of Intestinal Microbiota by qPCR
  • 06:33Results: Quantification of Intestinal Microbiota by qPCR after Oral Antibiotic Treatment
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

Tags

Keywords: Quantitative PCRMicrobiotaOral Antibiotic TreatmentInflammatory Bowel DiseaseAntibiotic CocktailDrinking WaterStool SampleWeight LossMicrobial Dysbiosis

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