Aseptic Technique Protocol
Aseptic Technique Protocol - Related Videos
10:48
Aseptic Technique in Environmental Science
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Aseptic technique is a fundamental skill widely practiced in the field of environmental microbiology that requires a balance of mindfulness and practice in the laboratory. Proper use of this technique reduces the likelihood of bacterial or fungal contamination of reagents, culture media, and environmental samples. Aseptic technique is also vital to ensure data integrity and...
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
12:40
A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia
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2022
The present paper describes a modified technique for heterotopic vascularized cardiac transplantation with updated aseptic technique, analgesia, and anesthesia.
09:46
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
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Cited by 9 •
2018
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
FAQs
Cross-contamination can introduce unwanted microorganisms into cultures, specimens, instruments, or clinical environments, making observed results harder to interpret. In immunology and infection research, contamination may affect cell cultures, pathogen studies, immune-cell assays, and specimen processing at the same time. Limiting contact between sterile components and nonsterile surfaces therefore supports experimental accuracy, reproducibility, and safer handling of biological materials.
Hand hygiene, personal protective equipment, surface disinfection, sterilized materials, and careful handling address different opportunities for microbial entry. No single measure provides the full control needed during laboratory or clinical work. Their combined use reduces exposure and contact with nonsterile surfaces, helping preserve sample integrity while also protecting researchers and patients from unwanted biological contamination.
Contact with a nonsterile surface creates an opportunity for unwanted microorganisms to reach a sterile component, culture, specimen, or instrument. Careful handling limits that transfer pathway by reducing unnecessary exposure during work. This principle matters because even when other controls are present, careless contact can compromise biological samples and weaken confidence in infection or host-response findings.
Effectiveness depends on consistent attention to several linked practices: hand hygiene, suitable personal protective equipment, disinfected surfaces, sterilized materials, and handling that limits exposure. Weakness in any of these areas can increase opportunities for contamination or cross-contamination. Maintaining the practices together supports reliable sample processing, protects personnel and patients, and improves the reproducibility of experimental results.
A basic procedure combines hand hygiene with appropriate personal protective equipment, prepares or maintains disinfected work surfaces, uses sterilized materials, and handles components to minimize exposure. The process also requires avoiding contact between sterile items and nonsterile surfaces. Applying these elements consistently during preparation and processing helps preserve cultures and specimens while reducing contamination-related variability.
Researchers apply these practices during cell culture, pathogen studies, immune-cell assays, and specimen processing. Each setting depends on maintaining the condition of biological materials so that measured responses reflect the intended experiment rather than unwanted microbial exposure. The approach also supports safer laboratory work and helps produce findings that can be interpreted more confidently in studies of infection and host responses.
Consistent practice improves the reliability of cultures, specimens, and assay materials by reducing contamination and cross-contamination. As a result, experimental differences are less likely to reflect unwanted microorganisms introduced during handling. This strengthens reproducibility and makes observations from pathogen studies, immune-cell assays, and host-response investigations easier to interpret as meaningful biological findings.