Bv-2 Cell Culture
Bv-2 Cell Culture - Related Videos
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Cited by 17 •
2015
Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.
11:33
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
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Cited by 5 •
2019
Here we present a protocol for Ca2+ imaging in neurons and glial cells, which enables the dissection of Ca2+ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca2+ indicators.
11:24
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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Cited by 14 •
2015
Zinc-finger domains are intrinsically cell-permeable and capable of mediating protein delivery into a broad range of mammalian cell types. Here, a detailed step-by-step protocol for implementing zinc-finger technology for intracellular protein delivery is presented.
07:50
Preparation of 2-dGuo-Treated Thymus Organ Cultures
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Cited by 9 •
2008
This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
FAQs
Exposure to lipopolysaccharide or inflammatory cytokines can shift Bv-2 cells into an activated signaling state. The resulting pathway activity is assessed through downstream mediators such as nitric oxide, cytokines, and chemokines. Measuring these outputs helps connect an experimental stimulus with the inflammatory response rather than treating activation as a single, nonspecific event.
The value of Bv-2 cell culture lies in its reproducibility as an intermediate experimental system. Investigators can examine responses under controlled laboratory conditions before moving to primary cells or animal studies. This staged approach supports initial testing of inflammatory mechanisms and candidate interventions while keeping the experimental setting more defined than later biological models.
Because microglia are the resident immune cells of the central nervous system, Bv-2 responses provide a cellular perspective on neuroinflammation. Changes in nitric oxide, cytokines, or chemokines can be interpreted as outputs of microglial immune activity. This makes the model relevant when a research question connects immune signaling with infection or inflammation in nervous tissue.
A basic experiment begins by maintaining the cells under controlled conditions and allowing them to adhere to a growth surface. Researchers then introduce a stimulus, such as lipopolysaccharide or an inflammatory cytokine, and examine mediator responses. This sequence separates cell maintenance, stimulation, and response assessment, helping link the treatment to observed immune activity.
Host-pathogen experiments can use the culture to examine how microglial immune activity changes in response to infection-related questions. Investigators can focus on activation-associated nitric oxide, cytokine, and chemokine responses while working in a controlled in vitro setting. Results can clarify inflammatory patterns and help determine whether follow-up work in primary cells or animals is warranted.
Potential anti-inflammatory interventions can be examined by comparing mediator responses under stimulated conditions with responses after the intervention is introduced. Reduced or otherwise altered nitric oxide, cytokine, or chemokine outputs may indicate that the treatment affects inflammatory signaling, although the culture serves as an initial model before evaluation in primary cells or animal studies.