Conventional Dendritic Cells
Conventional Dendritic Cells - Related Videos
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
FAQs
Exposure to inflammatory signals promotes maturation, which shifts conventional dendritic cells from a peripheral antigen-capturing state toward a role suited for immune communication. Mature cells migrate to draining lymph nodes, where processed peptide-MHC displays and costimulatory signals engage naïve T cells and initiate pathogen-specific adaptive responses.
Captured pathogens or cellular material are processed into peptide fragments. The fragments are then displayed on major histocompatibility complex molecules, creating the antigen-presenting signal that allows naïve T cells in draining lymph nodes to recognize infection-related material. This processing step connects peripheral sampling with the specificity of the ensuing adaptive immune response.
Costimulatory signals accompany antigen presentation when conventional dendritic cells activate naïve T cells. Their inclusion means the response is shaped by more than peptide display alone: the dendritic cell supplies coordinated signals in the lymph node that help direct a pathogen-specific adaptive response. This makes costimulation a key feature to examine when studying infection or vaccination.
Migration to draining lymph nodes places processed antigen in the setting where naïve T-cell activation occurs. This movement matters because peripheral capture and inflammatory maturation would not by themselves describe the downstream adaptive response. Following migration helps connect local infection-related events with the initiation of pathogen-specific immunity in lymphoid tissue.
A study can follow a linked sequence: conventional dendritic cells capture pathogens or cellular material in peripheral tissues, encounter inflammatory signals, mature, migrate to draining lymph nodes, and present processed peptides with costimulatory signals to naïve T cells. This sequence organizes observations across infection sites and lymphoid tissue without separating innate and adaptive phases.
Examining development and subsets adds context to how conventional dendritic cells participate in immune responses. The overview identifies these features, together with interactions with microbes, as essential for understanding infection, immune regulation, vaccine responses, and immunotherapy design. Researchers can therefore use this context to relate cell biology to broader immune outcomes.
Conventional dendritic cell research informs vaccine responses and immunotherapy because these cells influence how antigen presentation, costimulation, and pathogen-specific T-cell activation are connected. It also provides context for immune regulation during infection. Examining these relationships helps explain why dendritic-cell interactions with microbes matter when designing immune-based interventions.