Ex Vivo Cell Culture
Ex Vivo Cell Culture - Related Videos
08:33
An Ex vivo Culture System to Study Thyroid Development
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Cited by 14 •
2014
This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
08:39
Ex vivo Culturing of Whole, Developing Drosophila Brains
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Cited by 16 •
2012
This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.
07:44
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Cited by 9 •
2016
Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.
FAQs
Ex vivo cell culture supports controlled stimulus-response experiments by exposing primary cells to defined antigens, cytokines, or infectious agents. Investigators can then examine activation, signaling, cytokine production, pathogen replication, and host-cell responses. Separating the stimulus from the original organism helps link a measured response to a specific experimental condition.
Primary cells retain functions associated with the tissue and individual from which they were obtained, making their responses useful for studying physiologically relevant mechanisms. In immunology, this supports analysis of immune regulation and activation; in infection research, it enables assessment of how host cells respond to infectious agents under defined conditions.
Temperature, gases, nutrient-rich medium, and other environmental conditions must be controlled because they provide the context in which removed cells maintain their functions. If these conditions are appropriate, measured activation, signaling, or cytokine production can be interpreted in relation to the applied stimulus rather than an unspecified laboratory environment.
Measuring pathogen replication alongside host-cell responses allows investigators to consider both sides of an infection-related interaction. The same culture can reveal whether an infectious agent is associated with changes in cellular activation, signaling, or cytokine production. This combined view helps characterize host responses and mechanisms linked to disease progression.
Researchers obtain cells from a specific tissue or individual, maintain them in nutrient-rich medium under suitable temperature, gas, and environmental conditions, and apply a defined stimulus. They then measure outcomes such as immune-cell activation, signaling, cytokine production, pathogen replication, or host-cell responses to support a defined comparison.
It is useful when the goal is to study disease progression, immune regulation, or therapeutic responses using cells from a particular tissue or individual. The approach also supports controlled investigations of pathogen replication and host responses, allowing defined antigens, cytokines, or infectious agents to be examined in relation to immune-cell behavior.
In immunology and infection research, the method provides a cellular setting for disease-related questions. Investigators can examine how immune cells activate, signal, and produce cytokines, while also tracking pathogen replication and host-cell responses. These readouts help connect immune regulation with infection processes and potential therapeutic responses.