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An Ex vivo Culture System to Study Thyroid Development
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An Ex vivo Culture System to Study Thyroid Development

Article DOI: 10.3791/51641
June 6th, 2014 •
1, 1, *1, *1
1Pole of Cell Biology, Université catholique de Louvain & de Duve Institute
* These authors contributed equally

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June 6th, 2014

This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.

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