Intracranial Cannula Implantation
Intracranial Cannula Implantation - Related Videos
04:01
Cannula Implantation into the Cisterna Magna in a Mouse Model
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2025
Source: Xavier, A. L. R., et al. Cannula Implantation into the Cisterna Magna of Rodents. J. Vis. Exp. (2018)This video demonstrates the procedure for implanting a cannula into the cisterna magna of an anesthetized mouse. The technique involves guiding a needle-mounted cannula through the dura into the inverted triangular cisterna magna and securing it for targeted delivery into the cerebrospinal fluid.
05:22
Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice
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Cited by 7 •
2023
Central nervous system (CNS) tumors are the leading cause of cancer-related death in children, and locoregional immune-based therapies are increasingly being tested for patients in clinical trials. This protocol describes methods for locoregional cannula implantation in mice for the preclinical evaluation of immunotherapeutic infusions targeting CNS tumors.
10:13
Cannula Implantation into the Cisterna Magna of Rodents
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Cited by 134 •
2018
Here we describe a protocol to perform cisterna magna cannulation (CMc), a minimally invasive way to deliver tracers, substrates and signaling molecules into the cerebrospinal fluid (CSF). Combined with different imaging modalities, CMc enables glymphatic system and CSF dynamics assessment, as well as brain-wide delivery of various compounds.
08:06
Direct Cannula Implantation in the Cisterna Magna of Pigs
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Cited by 1 •
2021
This article presents a step-by-step protocol for the direct cannula implantation in the cisterna magna of pigs.
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
FAQs
Stereotaxic guidance links the cannula's position to predetermined coordinates, allowing investigators to target a defined brain region or cerebral ventricle rather than access the central nervous system indiscriminately. That spatial control is important when comparing localized neuroimmune effects with broader ventricular exposure. It also supports consistent delivery or sampling across experimental conditions.
Placement determines the compartment available for experimental access. Positioning the tube in a defined brain region supports localized infusion or measurement, whereas placement in a cerebral ventricle provides access to the ventricular environment and can support cerebrospinal fluid collection. This distinction helps align the implantation site with the biological question being studied.
Securing the cannula helps preserve its predetermined position while the surrounding tissue recovers. Maintaining that placement is essential for repeated infusion or sampling because movement could change the accessed site and complicate interpretation. Allowing recovery also helps investigators study subsequent immune, infectious, or treatment-related responses under more controlled experimental conditions.
The targeted location, the choice between regional and ventricular access, and the ability to repeat infusion or sampling all shape the information obtained. These factors determine whether investigators examine localized central nervous system responses, cerebrospinal fluid-related changes, or treatment effects over repeated measurements. Careful alignment between access conditions and the research question improves experimental control.
The procedure begins with stereotaxic guidance to identify predetermined coordinates, followed by creation of a small cranial opening. The narrow tube is positioned in the selected brain region or cerebral ventricle and then secured. After the surrounding tissue recovers, the implanted cannula can support controlled infusion or sampling during later experimental observations.
In this research context, investigators can use the implanted access route to deliver immune modulators, pathogens, or therapeutics directly to the central nervous system. They can then examine neuroimmune signaling, inflammation, central nervous system infection, or treatment responses. The approach helps separate effects associated with controlled central delivery from observations made without localized access.
Repeated access allows researchers to follow central nervous system processes through controlled delivery or collection over the course of an experiment. Sampling can support cerebrospinal fluid-related measurements, while infusion can test responses to immune modulators, pathogens, or therapeutics. Together, these capabilities help evaluate localized biological changes and responses to treatment.