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Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

DOI:
10.3791/64886-v

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05:22 min

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February 24, 2023

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, , , , , , , ,
1Division of Oncology,Children’s Hospital of Philadelphia, 2Center for Data Driven Discovery in Biomedicine,Children’s Hospital of Philadelphia, 3Division of Neurosurgery,Children’s Hospital of Philadelphia, 4Ben Towne Center for Childhood Cancer Research,Seattle Children’s Research Institute, 5Department of Pediatrics, Seattle Children’s Hospital,University of Washington

Chapters

  • 00:04Introduction
  • 00:48Mouse Preparation and Surgery Procedure
  • 03:47CAR T Cell Infusion
  • 04:36Results: Efficacy of CAR T Cell Therapy
  • 04:53Conclusion

Summary

Automatic Translation

Automatic Translation

Central nervous system (CNS) tumors are the leading cause of cancer-related death in children, and locoregional immune-based therapies are increasingly being tested for patients in clinical trials. This protocol describes methods for locoregional cannula implantation in mice for the preclinical evaluation of immunotherapeutic infusions targeting CNS tumors.

Tags

Keywords: Intracranial CannulaCAR T CellLocoregional DeliveryIntraventricular CatheterStereotactic SurgeryStereotaxic ApparatusCranial LandmarksAcrylic ResinRepeated DosingNeuroscience Research

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