Micro-culture System
Micro-culture System - Related Videos
12:39
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
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Cited by 7 •
2008
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
09:27
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Cited by 86 •
2010
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
09:47
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
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Cited by 16 •
2015
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.
02:53
Developing a Micro-Tissue-Engineered Neural Network Using a Hydrogel-Based Micro-column
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2025
Source: Struzyna, L. A. et. al., Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling. J. Vis. Exp. (2017)This video demonstrates the development of micro-tissue-engineered neural networks using a hydrogel micro-column with an extracellular matrix core. Seeded neuronal aggregates adhere, extend projections, and form structured neural networks, contributing to advancements in neurodevelopment and...
12:21
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
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Cited by 3 •
2013
We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or reagents.
FAQs
Compartmentalization creates defined local environments in which researchers can control cell number, nutrients, oxygen, and exposure conditions. This reduces variation between experimental units and helps separate effects caused by biological interactions from those caused by uncontrolled culture conditions. The resulting measurements can more consistently reflect growth, viability, signaling, or interactions between cells and microorganisms.
Cell number, nutrient availability, oxygen, and exposure conditions are central variables because each can alter how cells or microorganisms grow and respond. Adjusting these factors within small, defined volumes allows researchers to examine their effects systematically. Such control is especially useful when interpreting immune responses, pathogen replication, or signaling in mixed biological communities.
Mixed communities allow host cells, immune cells, and microorganisms to be examined together rather than as isolated populations. In this setting, researchers can investigate host-pathogen encounters, cell-to-cell interactions, and immune signaling under controlled conditions. The approach connects cellular behavior with measurable outcomes such as cytokine production or pathogen replication, supporting more integrated infection models.
A study begins by placing the selected cells, microorganisms, or mixed community into defined small-volume compartments. Researchers then regulate relevant conditions, including cell number, nutrients, oxygen, and exposure, while running comparable experimental units in parallel. Measurements can subsequently focus on growth, viability, signaling, cell-to-cell interactions, cytokine production, or pathogen replication.
Researchers may choose this approach when they need controlled models of host-pathogen encounters or want to evaluate immune-cell responses under defined exposure conditions. The format also suits experiments requiring multiple parallel conditions, such as comparisons of cellular responses or therapeutic screening. Using smaller volumes can reduce sample and reagent requirements while supporting reproducible analysis.
Depending on the experimental design, the system can provide measurements of growth, viability, signaling, cell-to-cell interactions, cytokine production, or pathogen replication. These readouts help characterize how immune cells respond to microorganisms and how infection-related interactions change under controlled conditions. Parallel experiments can further support scalable infection models and therapeutic screening.