Murine Lung Analysis
Murine Lung Analysis - Related Videos
13:32
Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
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Cited by 29 •
2014
Hypoxic pulmonary vasoconstriction (HPV) is an important physiological phenomenon by which at alveolar hypoxia lung perfusion is matched to ventilation. The major vascular segment contributing to HPV is the intra-acinar artery. Here, we describe our protocol for the analysis of HPV of murine pulmonary vessels with diameters of 20-100 μm.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
03:06
Preparation of a Murine Oropharyngeal Aspiration Model to Establish Bacterial Lung Infections
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2026
Source: Nielsen, T. B., et al., Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia. J. Vis. Exp. (2018)This video demonstrates the procedure of delivering bacteria through the oropharynx into the lungs of an anesthetized mouse to induce lung infection and trigger an immune response.
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
FAQs
The approach links structural injury, recovered lung-fluid cells, and molecular signals into a combined picture of pulmonary disease. Histology can show tissue damage, while bronchoalveolar lavage and flow cytometry characterize recruited immune cells. Molecular assays then indicate inflammatory signaling or pathogen burden, helping researchers relate visible pathology to underlying host-defense activity.
Each method examines a different level of the response. Histology focuses on lung structure and injury; bronchoalveolar lavage provides access to cells and material in the airspaces; flow cytometry identifies immune-cell populations; and molecular assays assess pathogen burden or inflammatory signaling. Combining these readouts reduces reliance on a single indicator of disease.
Standardized analysis makes measurements more comparable across disease models and experimental groups. Consistent assessment of tissue structure, recovered cells, pathogen burden, and inflammatory signals helps distinguish biological differences from variation caused by the analysis itself. This strengthens interpretation when researchers compare respiratory infections, host responses, or the effects of different interventions.
Evidence can come from several coordinated findings rather than one measurement alone. Tissue injury on histology, increased immune-cell recruitment in lung samples or lavage, and altered inflammatory signaling together support an inflammatory response. Measuring pathogen burden alongside these features helps researchers determine whether inflammation accompanies infection and how strongly the host response affects the lung.
A study generally examines both lung tissue and lung fluid, then applies complementary measurements to those materials. Researchers assess structure with histology, characterize recovered immune cells with flow cytometry, and use molecular assays to evaluate pathogen burden or inflammatory signaling. The resulting measurements can be compared across experimental groups to relate infection or treatment to pulmonary outcomes.
It is useful when an intervention must be judged by more than pathogen burden alone. Murine lung analysis can compare treated and untreated groups for tissue injury, immune-cell recruitment, inflammatory signaling, and pathogen levels. This combination helps show whether a vaccine or antimicrobial treatment is associated with improved pulmonary responses and reduced disease-related changes.