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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
 

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Article doi: 10.3791/52257
November 21st, 2014 •
1, 1
1Department of Pathology, University of California, San Diego

Summary
Automatic Translation
November 21st, 2014

This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.

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