Proviral Reporter Integration
Proviral Reporter Integration - Related Videos
14:27
CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
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Cited by 6 •
2018
We present a genome engineering workflow for the generation of new in vitro models for HIV-1 infection that recapitulate proviral integration at selected genomic sites. Targeting of HIV-derived reporters is facilitated by CRISPR-Cas9-mediated, site-specific genome manipulation. Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided.
10:20
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
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Cited by 2 •
2008
Current HIV-1 strategies act to suppress the viral life cycle but do not effectively eradicate infection. Here, we demonstrate that an engineered recombinase can efficiently excise integrated HIV-1 proviral DNA from the genome of infected cells.
05:33
Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System
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Cited by 1 •
2025
Integrated image management, artificial intelligence (AI), and reporting systems have revolutionized diagnostic pathology practice. In this paper, we introduce FlexLIS, a state-of-the-art system that enables AI to assist pathologists in performing histopathology image assessments and generating diagnostic reports.
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
01:24
Data Reporting and Recording
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2023
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
FAQs
Once the proviral construct is incorporated into host chromosomes, its viral regulatory elements determine when the reporter gene is transcribed. Reporter output therefore changes with transcriptional or replication-related activity rather than simply indicating that viral DNA is present. This linkage allows experiments to monitor functional viral activity within infected or engineered cells.
The approach compares reporter activity under conditions where integrated viral sequences remain transcriptionally quiet with conditions that promote viral gene activity. Latent proviruses are associated with little or no reporter signal, whereas activation produces a measurable fluorescent or luminescent response. This contrast supports analysis of latency, reactivation, and changes in viral activity.
Both fluorescent and luminescent reporters provide measurable signals linked to viral gene activity, but they represent different readout formats for monitoring that activity. Their sensitivity and quantitative behavior can support comparisons among experimental conditions, such as untreated and antiviral-treated cells. The selected signal should match whether the study emphasizes visualization, measurement, or both.
A typical workflow begins with infection or engineered delivery of the reporter-containing viral construct into host cells. After the construct integrates into cellular chromosomes, researchers measure reporter output under defined experimental conditions. They can then compare signals associated with viral activity, reduced activity, reactivation, antiviral exposure, or immune-mediated suppression without relying only on indirect observations.
Researchers can apply this strategy when they need a quantitative indicator of how treatment changes viral activity in cells or experimental models. Reporter output can be compared before and after antiviral exposure, helping identify reduced viral gene activity or altered reactivation. The same framework also supports evaluation of immune-mediated suppression as an experimental outcome.
In immunology and infection studies, integrated reporter systems connect cellular or immune conditions with measurable changes in viral activity. They can be used to follow infection dynamics, examine transitions between latent and active states, and assess suppression by immune responses. These measurements provide a practical way to relate experimental conditions to proviral behavior.