Streptavidin Staining
Streptavidin Staining - Related Videos
03:41
Analyzing Influenza Virus Internalization Using a Streptavidin Blocking Assay
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2026
Source: Pohl, M. O. & Stertz, S. Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry. J. Vis. Exp. (2015)This video demonstrates the use of a streptavidin blocking assay to distinguish surface-bound from internalized influenza virus in human lung epithelial cells. Comparing fluorescence signals shows that a higher intensity after incubation indicates successful viral internalization.
04:34
An Immunohistochemistry Staining for the Detection of Rabies Virus Antigens
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2025
This video demonstrates immunohistochemistry staining for rabies virus antigen detection in infected mouse brain tissue. The process includes deparaffinization and alcohol treatment, followed by proteolytic treatment to enhance antigen accessibility. Immunohistochemistry staining reveals red precipitates, confirming the presence of rabies antigens in the tissue section.
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
03:27
Immunofluorescent Staining of Sialoglycoproteins in Neural Progenitor Cells and Neurons
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2025
This video demonstrates the biotinylation and fluorescent labeling of modified sialoglycoproteins in primary neural stem and progenitor cells, as well as neurons, and their visualization using fluorescence microscopy.
08:07
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
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2025
This article describes a protocol for studying DNA-protein interactions using a streptavidin-based biolayer interferometry (BLI) system. It outlines the essential steps and considerations for utilizing either basic or advanced binding kinetics to determine the equilibrium binding affinity (KD) of the interaction.
FAQs
Its value comes from the interaction’s combination of high specificity and strong binding. A biotin label placed on an antibody, antigen probe, or secondary reagent provides a defined attachment site for the streptavidin conjugate. This links the selected target to an enzyme or fluorescent dye, allowing the assay to convert molecular recognition into a visible or measurable signal.
The conjugate determines how the bound label becomes detectable. Enzyme-linked streptavidin produces a signal through substrate conversion, whereas fluorescently linked streptavidin is detected after illumination. Consequently, the same biotin-dependent recognition strategy can support different readouts, including microscopy-based visualization, immunohistochemistry, immunofluorescence, and flow cytometry, depending on the experimental format.
The labeled molecule is selected according to the target and assay design. A biotinylated antibody can recognize an immune or pathogen-associated marker, while a biotinylated antigen probe or secondary reagent can connect other binding events to detection. This arrangement preserves target specificity while positioning biotin where the streptavidin-linked enzyme or dye can generate the final signal.
The detection signal can be examined spatially or quantitatively, depending on the platform. Microscopy and immunohistochemistry reveal where labeled targets occur in cells, tissues, or microbial samples, while flow cytometry supports analysis of labeled cells. These complementary uses allow investigators to localize pathogens or immune markers and assess cellular responses within the chosen sample type.
A typical workflow first allows a biotinylated antibody, antigen probe, or secondary reagent to bind its target. The sample is then exposed to streptavidin carrying an enzyme or fluorescent dye, which attaches to the available biotin. Detection follows through substrate conversion for enzyme labels or illumination for fluorescent labels, producing the assay signal.
This approach is useful when experiments must identify immune markers, detect or localize pathogens, or evaluate cellular responses. It can be applied to cells, tissues, or microbial samples through immunofluorescence, immunohistochemistry, flow cytometry, or microscopy-based analysis. The selected format depends on whether the study prioritizes spatial localization, cell-based measurement, or diagnostic assay detection.