Adding The Opposite

Adding the opposite is a mathematical strategy in which a number is combined with its additive inverse, producing zero and canceling the original value. The opposite of a number has the same distance from zero on the number line but lies in the other direction, so a + (−a) = 0; this cancellation can simplify expressions and preserve equality when applied to both sides of an equation. In arithmetic and algebra, the method helps convert subtraction into addition, remove terms, and isolate unknowns when solving equations. It also supports reasoning with integers, rational numbers, and signed quantities in scientific models.

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Education

JoVE Science Education - Psychology

Marginal Dishonesty: The Adding-to-10 Task

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2023

Source: Julian Wills & Jay Van Bavel—New York University Classical economic theory asserts that people are rational and self-interested. In addition to seeking wealth and status, people are motivated by other goals. As a result, financial motives can sometimes be dwarfed by other internal needs, such as maintaining a positive self-concept or affiliating with other group members. Ethical dilemmas, such as the temptation to cheat on taxes, can result when these motives are in conflict. On...

Research

JoVE Journal - Biochemistry

Identification of Fatty Acids in Bacillus cereus

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Cited by 9 •

2016

We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).

Research

JoVE Journal - Cancer Research

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

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Cited by 4 •

2025

This protocol details the glycomics-guided glycoproteomics method, an integrated omics technology that offers comprehensive insights into the heterogeneous glycoproteome in complex tumor microenvironments required to better understand the glycobiology of cancers.

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

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Cited by 6 •

2019

Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

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Cited by 3 •

2024

The presented method describes the generation of a CRISPR-mediated gene knockout in the human embryonic stem cell (hESC) line H9, which stably expresses sgRNAs targeting the L2HGDH gene using a highly efficient lentiviral-mediated gene delivery system.

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