Granulocyte Count
Granulocyte Count - Related Videos
04:59
An Assay to Assess Phagocytosis and Oxidative Burst Activity In Granulocytes and Monocytes
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2025
This video demonstrates the assay to confirm phagocytic activity by cells in human blood via flow cytometry. The granulocytes and monocytes confirm their phagocytic activity by showing green and red fluorescence confirming the presence of engulfed bacteria and oxidative burst activity respectively.
11:29
Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
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Cited by 5 •
2016
The purpose of this manuscript is to present a method for measuring monocyte and granulocyte phagocytosis and oxidative burst activity in human blood samples.
10:19
Using a Hemacytometer to Count Cells
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2023
Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant data. Therefore, learning how to count cells is a particularly essential technique for any successful biomedical scientist. The most common way to count cells is by using a hemacytometer - an instrument that bears two laser-etched grids, which aid in the enumeration of an aliquot cells under a simple light microscope. This data can then be used to...
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
12:23
Granulocyte-dependent Autoantibody-induced Skin Blistering
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2012
In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human epidermolysis bullosa acquisita (EBA)1.
FAQs
The absolute neutrophil count combines the total white blood cell count with the percentage of neutrophils to estimate the number of circulating neutrophils available for immediate defense against infection. This makes it especially useful when clinicians need to assess infection risk more specifically than by reviewing the overall granulocyte measurement alone.
An unusually low result may indicate reduced immune defense or treatment-related immune suppression, while an elevated result can occur in association with infection or inflammatory conditions. Interpretation depends on which granulocyte population is changing, because neutrophils, eosinophils, and basophils provide different information about possible bacterial or fungal infection, allergy, inflammation, or bone marrow disease.
A complete blood count with differential separates the granulocyte result into neutrophils, eosinophils, and basophils rather than treating all white blood cells as one group. That distinction helps connect the laboratory pattern with different clinical concerns, including infection, allergic or inflammatory conditions, bone marrow disorders, and suppression of immune cells by treatment.
Testing begins with a blood sample analyzed through a complete blood count with differential. The differential identifies the proportions or measured results for neutrophils, eosinophils, and basophils, while the absolute neutrophil count is derived from the total white blood cell count and neutrophil percentage. Clinicians then interpret these findings in relation to suspected disease or treatment effects.
Clinicians may use this testing when evaluating possible bacterial or fungal infection, allergic or inflammatory disease, or a disorder affecting bone marrow. It is also useful when treatment may suppress immune function. In those settings, abnormal results can indicate whether additional evaluation is needed and provide a laboratory basis for tracking immune status.
Repeated measurements allow clinicians to observe whether granulocyte results change during therapy rather than relying on a single blood test. A rising or falling pattern may help assess the patient’s response to treatment or identify treatment-related immune suppression. Abnormal trends can also guide decisions about further testing and continued clinical evaluation.