In Vivo Approaches
In Vivo Approaches - Related Videos
08:51
An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
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Cited by 6 •
2014
Multi-component protein complexes play crucial roles during cellular function and development. Here we describe a method used to isolate native protein complexes from Drosophila embryos after in vivo crosslinking followed by purification of the crosslinked complexes for subsequent structure-function analysis.
13:04
In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
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Cited by 10 •
2015
We outline a protocol that implements both in vivo and ex vivo approaches to study ovarian cancer colonization of peritoneal adipose tissues, particularly the omentum. Furthermore, we present a protocol to quantitate and analyze immune cell-structures in the omentum known as milky spots, which promote metastases of peritoneal adipose.
08:13
A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
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Cited by 11 •
2012
A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.
12:17
The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
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Cited by 18 •
2017
We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.
09:47
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
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Cited by 6 •
2012
A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.
FAQs
Intact tissues and organ systems preserve interactions that can be missed in isolated molecular or cellular studies. A treatment may be altered by metabolism, influenced by immune responses, or affected by communication among organs. Measuring outcomes in this integrated setting helps determine whether an observed biological effect persists under whole-organism conditions and better reflects therapeutic performance.
Pharmacokinetic measurements show how an administered treatment behaves in the organism, while toxicity assessments identify harmful effects under defined conditions. Together, these outcomes connect exposure with both intended and adverse responses. This information helps researchers judge whether a treatment reaches relevant biological systems, produces a useful effect, and has a safety profile suitable for further evaluation.
Findings from molecular and cellular studies may indicate how a treatment should work, but they do not fully capture organism-level responses. In vivo research tests whether those findings translate into changes in physiology, disease progression, treatment efficacy, or safety. This connection helps refine therapeutic strategies when whole-organism results differ from predictions based on simpler experimental systems.
Metabolism, immune responses, and interactions among organs can all influence how an intervention behaves and what effects it produces. These factors may alter treatment exposure, physiological responses, toxicity, or disease progression. Considering them is important when interpreting results, because an outcome reflects the combined activity of the intervention and the organism’s integrated biological systems.
A study generally begins by administering a drug, treatment, or biological intervention to an animal or human participant under defined conditions. Researchers then measure selected outcomes, such as physiological responses, pharmacokinetics, toxicity, or disease progression. Comparing these measurements with the study’s intended therapeutic goals allows investigators to evaluate effects in the context of the living organism.
Medical researchers use these approaches when they need evidence about treatment efficacy and safety beyond molecular or cellular findings. The results can support evaluation before clinical use, reveal how metabolism and organ interactions influence outcomes, and guide refinement of therapeutic strategies. They also help assess disease progression and determine whether an intervention produces meaningful physiological responses.