Simple and Robust <em>in vivo</em> and <em>in vitro</em> Approach for Studying Virus Assembly

Sonali Chaturvedi; Bongsu Jung; Sharad Gupta; Bahman Anvari; A.L.N. Rao

doi:10.3791/3645

Journal

/

Immunology and Infection

/

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

12,150 Views

•

09:47 min

•

March 01, 2012

DOI:

10.3791/3645-v

DOI:

10.3791/3645-v

•

09:47 min

March 01, 2012

•

4 Views

Sonali Chaturvedi¹, Bongsu Jung², Sharad Gupta², Bahman Anvari², A.L.N. Rao¹

¹Department of Plant Pathology and Microbiology,University of California, Riverside , ²Department of Bioengineering,University of California, Riverside

Summary

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.