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Immunology and Infection

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Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Article DOI: 10.3791/3645
March 1st, 2012 •
1, 2, 2, 2, 1
1Department of Plant Pathology and Microbiology, University of California, Riverside, 2Department of Bioengineering, University of California, Riverside

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March 1st, 2012

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.

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