/
/
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 03:16Delivery and Expression of Functional Viral Genome Components to Plant Cells by Agroinfiltration
- 06:00Preparation of Capsid Protein Subunits for in vitro Assembly
- 07:07In vitro Assembly of RNA-containing Virions
- 08:48Representative Viral Assembly Results
- 09:22Conclusion
A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.
Tags
Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration
