Intestinal Tube Perfusion
Intestinal Tube Perfusion - Related Videos
06:01
Using An In Vitro Tissue Perfusion System to Detect the Functional Activities of Isolated Intestinal Tubes in Real Time
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2024
We present a method for isolating rat intestinal tubes and assessing the impact of drugs on their tension, frequency, and amplitude in vitro. This method offers a valuable approach for researchers investigating intestinal tubes.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
08:10
A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
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Cited by 42 •
2013
The article describes a methodology for the production of an acellular matrix from rat intestine. The derivation of intestinal scaffolds is important for future applications in tissue engineering, stem cell biology and drug testing.
FAQs
Controlled flow conditions determine how long the test solution contacts the intestinal mucosa and help make results comparable across experiments. By maintaining a defined exposure environment, researchers can relate changes in the collected solution to intestinal handling rather than uncontrolled differences in delivery. This improves evaluation of absorption, permeability, transport, and secretion within the studied segment.
The collected downstream solution is compared with the material introduced into the intestinal segment. Changes in its composition provide evidence that substances were absorbed, transported, secreted, or otherwise altered during mucosal contact. These measurements connect the observed chemical changes with intestinal function and help characterize how the gut handles a test compound under the selected experimental conditions.
The method examines the intestinal wall as substances pass through a defined segment, allowing investigators to assess several related processes rather than drug disappearance alone. Absorption concerns movement from the intestinal contents, permeability reflects passage across the wall, and transport or secretion describe directed handling by the intestine. Together, these measurements provide a more complete view of mucosal function.
A typical experiment establishes a defined intestinal segment, introduces the test solution through a tube, maintains controlled flow while the solution contacts the mucosa, and collects the downstream material. Researchers then assess how the collected composition differs from the starting solution. This sequence creates a controlled comparison between input and output for evaluating intestinal handling.
Pharmacology researchers can use the approach to examine how a candidate compound interacts with the intestinal wall and how efficiently it may be absorbed. The resulting information supports evaluation of intestinal permeability and transport, which are important for understanding medication performance. It can therefore contribute to dose evaluation and decisions about how a formulation should be developed.
In medicine and pharmacology, the technique supports formulation development, dose evaluation, and investigation of gastrointestinal disorders. It links intestinal physiology with medication performance by showing how a controlled intestinal environment handles a test substance. This makes the method useful when researchers need experimental information about drug behavior or altered intestinal function rather than relying only on final clinical outcomes.