Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

The overall goal of this experiment is to genetically modify the murine myeloid 32D-G-CSF-R cells by over expressing or silencing the gene of interest and determining the effect of these changes on proliferation and neutrophilic differentiation of the cell line. This method can help to answer key questions in the field of hematology such as how your protein of interest can be involved in growth and differentiation of myeloid precursor cells. The main advantage of this cell line is that it possesses unlimited proliferation capacity, is susceptible to genetic manipulations, the cost is relatively low, and allows a degree of biological simplification required in certain experimental approaches.

Demonstrating the procedure today will be Petr Danek, a PhD student in my laboratory. Prepare 250 milliliters of RPMI-1640 Medium supplemented with heat-inactivated 10%fetal bovine serum and 10 nanograms per milliliter of murine interleukin-3. Then prepare 50 milliliters of differentiation medium.