Neuronal Cell Clusters

Neuronal cell clusters are organized groups of interconnected neurons that coordinate information processing within the nervous system, making them important for understanding neural function and disease. Their activity arises through synaptic communication, in which electrical impulses trigger neurotransmitter release and influence neighboring cells, while shared connectivity and local network properties shape collective responses. In medicine, studying neuronal cell clusters helps researchers investigate brain development, sensory processing, epilepsy, neurodegenerative disorders, and responses to therapeutic compounds. Cluster-based analyses of neural tissue and laboratory-grown neuronal models can reveal abnormal signaling patterns and support the development of diagnostic tools and targeted treatments.

Neuronal Cell Clusters - Related Videos

Research

JoVE Journal - Neuroscience

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

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Cited by 3 •

2017

This protocol describes large-scale reconstructions of selective neuronal populations, labeled following retrograde infection with a modified rabies virus expressing fluorescent markers, and independent, unbiased cluster analyses that enable comprehensive characterization of morphological metrics among distinct neuronal subclasses.

Research

JoVE Journal - Neuroscience
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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

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Cited by 36 •

2011

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

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Cited by 18 •

2008

Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

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Cited by 58 •

2014

We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s disease.

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

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Cited by 37 •

2009

Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in culture.

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