Optical Probe Placement

Optical probe placement is the deliberate positioning of a light-emitting and light-detecting device on, in, or near the body to obtain clinically useful measurements. It works by aligning the probe with a target tissue and maintaining an appropriate distance, angle, and contact pressure so emitted light interacts with tissue and the returning or transmitted signal can be recorded reproducibly. In medicine, careful placement supports optical spectroscopy, pulse oximetry, fluorescence assessment, and image-guided monitoring by improving signal quality and reducing artifacts from motion, ambient light, or inconsistent geometry. Standardized placement also strengthens comparisons across patients, procedures, and repeated measurements.

Optical Probe Placement - Related Videos

Research

JoVE Journal - Bioengineering

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

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Cited by 13 •

2012

Biosensors interface with complex, biological environments and perform targeted detection by combining highly sensitive sensors with highly specific probes attached to the sensor via surface modification. Here, we demonstrate the surface functionalization of silica optical sensors with biotin using silane coupling agents to bridge the sensor and the biological environment.

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

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Cited by 5 •

2010

This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.

Education

JoVE Science Education - Clinical Skills

Arterial Line Placement

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2023

Source: Sharon Bord, MD, Department of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA When monitoring patients, it is important to obtain values that are accurate and reliable. Blood pressure monitoring is one of the essential vital signs, and for a majority of patients, measuring it utilizing non-invasive techniques provides accurate values. However, there are situations in which the blood pressure requires more exact, specific, and reliable measurements.

Intraosseous Needle Placement

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2023

Source: Julianna Jung, MD, FACEP, Associate Professor of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA For unstable patients requiring urgent administration of medications, fluids, or blood products, establishing vascular access quickly is essential. However, there are many factors that can complicate placement of a peripheral intravenous cannula (PIV), and it is extremely common for PIV attempts to fail. PIV placement may be technically challenging in small...

Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics

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Cited by 1 •

2016

This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.

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